Research Associate - AAV Gene Therapy/Protein Engineering

Do you have a passion for science and take pride in excellent work? Would you love doing impactful research using cutting-edge technologies, while working together with highly motivated team members?

Who we are:

Dyno Therapeutics is a Cambridge based, VC-backed biotech startup that uses next-gen DNA technologies and machine learning to engineer Adeno-associated Virus (AAV) capsids for effective delivery of gene therapies.

What we offer you:

As a member of our new and quickly growing company, you’ll help us shape Dyno from the very beginning into a startup that not only takes its scientific mission seriously but also provides a positive and supportive workplace environment. And Dyno will have the opportunity to benefit from the insight, diversity, and talent that you’ll bring. Together, we’ll help turn gene therapy into reality.

Our mission:

Dyno wants to help transform the gene therapy universe. AAV capsids are the vector of choice when it comes to delivering transgenes in vivo for gene therapy and genome editing. Eclipsing all of its predecessors, AAV is at the forefront of gene delivery approaches both at the research level and at the clinical front. Dyno aims to make it even better. Doing so will enable treatment for millions of patients with currently incurable, often disabling and deadly diseases, and we invite you to become a part of that.

How?

Dyno has a transformative pipeline for AAV engineering that harnesses DNA library synthesis, high-throughput sequencing, and machine learning to improve AAV capsids and lift them above current fitness landscape boundaries into unexplored areas. While AAV is the current go-to method for gene delivery, there’s much room for improvement with regard to central factors like delivery efficiency, tissue and cell-type targeting, evading the immune system, and others. These limitations stand between the many significant advances achieved in gene therapy research and their translation into real-world, effective therapies. Our approach resolves these issues, generating next-gen AAV vectors that move gene therapies from the lab into clinics for the benefit of patients worldwide.

Where?

Dyno is located near Kendall Square in Cambridge within the dynamic LabCentral community, alongside other new companies that are turning ideas into reality, changing the biotech and medical scenes. We’ve recently joined this exciting hub and are thrilled to expand our Team with hardworking, highly qualified, highly motivated individuals.

General role: Under the guidance of senior researchers, you would support a number of stages in the Dyno pipeline, which includes i. generating complex AAV vector libraries from synthesized DNA and purifying large-scale plasmid DNA for AAV production, ii. generation and purification of AAV capsids using cultured mammalian cells for downstream in vitro and in vivo studies, including post-production quantification and quality assessment, and iii. extraction and purification of nucleic acids and proteins from mammalian cells and tissues, followed by detailed qualitative and quantitative analysis prior to high-throughput sequencing. Consistency, clever protocol optimization, and precision are key to Dyno’s workflow. This role calls for a broad Molecular Biology skillset and the ability to learn, develop, and optimize in vitro assays as the need arises. You would collaborate with scientists and computational biologists throughout Dyno – learning from and contributing to a diverse set of projects – and you would be responsible for understanding project goals and for creatively developing solutions to ensure their success.

Responsibilities:

  • Clone complex library assemblies for AAV production
  • Execute large scale DNA preparation protocols for transfection
  • Maintain and culture mammalian cells
  • Execute AAV production protocols: plating, transfection, harvest, purification, titration
  • Extract, purify, and enrich RNA/DNA/protein from mammalian cells and tissues
  • Follow and optimize ex vivonucleic acid quantification assays (e.g., RT-qPCR)
  • Perform and improve protein analysis methods (e.g., Bradford, Western Blots, ELISA)
  • Implement next-gen sequencing DNA preparation procedures (amplification/ligation)

Basic qualifications:

  • BA or MA in biology, biochemistry, biomedical engineering, or related fields
  • 2+ years hands-on wet lab experience
  • Ability to conduct advanced protocols and procedures precisely and consistently
  • Ability to document and maintain an organized account of lab work and results
  • Highly skilled in at least two of the following:
  • Cell culture: cell line maintenance, passaging, storage, etc.
  • Molecular biology: PCR, agarose gel electrophoresis and imaging, cloning expertise, bacterial transformation, culture, and plating, and plasmid preparation (miniprep, maxiprep)
  • Protein extraction and analysis: SDS-PAGE, Western blots (FLAG, HIS, HA, etc.)
  • DNA/RNA purification and analysis (qPCR, RT-qPCR, etc.)

Preferred qualifications:

  • Strong preference for individuals with a background in AAV production and purification
  • Focused experience in advanced DNA cloning methodologies
  • Focused experience in RNA/DNA quantification (qPCR) and quality control
  • Knowledge of oligo/primer/probe/gBlock design strategies
  • Advanced cell culture skills: Multi-layered flask cell growth, cell counting, transfection (PEI, Lipofectamine, etc.), antibiotic selection, virus titration, virus transduction
  • Cell analysis: staining, FACS, cell-sorting
  • Sequence design and analysis software skills, e.g., BLAST, Geneious, Benchling, etc.

We are an Equal Opportunity employer committed to a diverse workforce. We do not discriminate on the basis of race, religion, color, national origin, gender, sexual orientation, age, marital status, veteran status or disability.

Job Type: Full-time

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